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KMID : 0545119970070050363
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 5 p.363 ~ p.366
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Characterization of Doxorubicin-nonproducing Mutant, Nu3 of Streptomyces peucetius ATCC27952
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Hwang, Cheol Kyu
Lee, Hong Sub/Hong, Young Soo/Paek, Nam Soo/Kim, Tae Han/Lee, Jung Joon
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Abstract
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A doxorubicin-nonproducing mutant, Nu23 was selected from the mutagenesis of Streptomyces peucetius ATCC27952. Chemical and molecular biological analysis suggested that the mutant was blocked at the step between polyketide synthase and aklaviketon reductase in the biosynthesis of doxorubicin. Furthermore, the bioconversion experiment with the mutant revealed that 13-dihydrodaunorubicin is likely to be a biosynthetic intermediate.
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